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Key Results: We found support for a marked regional structure in A.
GENODIVE FORMAT DRIVERS
Finally, to reveal the drivers of genetic structure, we searched for associations between allele frequencies and spatial and climatic variables. Classical and model-based analysis was done to characterize the genetic structure within and between the sampled populations, complemented by genome size measurements and chromosomal counts. By using a ploidy-aware genotype calling based on likelihoods, we generated a dataset of 16,107 high-quality SNPs. In addition, we included two populations of A. tinctoria within its main distribution range in Greece and genotyped them using restriction site-associated DNA sequencing. Methods: We collected 14 populations of A. From a practical perspective, such investigation can inform biodiversity management strategies.
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Here, by employing a ploidy-aware framework, we investigated the genetic structure and its determinants in polyploid Alkanna tinctoria (Boraginaceae), an ancient medicinal herb that is the source of bioactive compounds known as alkannin and shikonin (A/S). In comparison with diploids, however, genetic variation and factors fostering genetic divergence remain poorly studied in polyploids due to analytical challenges.